R-plasmid mediated transfer of .BETA.-lactam resistance in Bacteroides fragilis.

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R-plasmid mediated transfer of beta-lactam resistance in Bacteroides fragilis.

We described plasmid mediated transfer of resistance to beta-lactam antibiotics between Bacteroides fragilis strains. Ampicillin-resistance was transferred from B. fragilis strain GAI-10150 to a B. fragilis strain JC-101 with a frequency of 10(-6)/input donor by a filter mating technique. A common plasmid band, named pBFKW1, was found in both the donor and the transconjugants. The plasmid was p...

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Beta-lactamase-mediated imipenem resistance in Bacteroides fragilis.

Imipenem has excellent antimicrobial activity owing in part to beta-lactamase stability. We found that only 2 of over 350 Bacteroides fragilis group clinical isolates were resistant to imipenem, with an MIC of more than 16 micrograms/ml. These two isolates from the Tufts Anaerobe Laboratory (TAL) were resistant to all other beta-lactam agents tested. The organisms were able to inactivate imipen...

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Physical characterization of Bacteroides fragilis R plasmid pBF4.

Bacteroides fragilis V479-1 has previously been shown to harbor a self-transmissible 27 X 10(6)-dalton plasmid (pBF4) which confers lincosamide-macrolide resistance. The present study has focused on the physical properties of pBF4. The plasmid was found to be present in 1 to 2 copies per chromosomal equivalent. pBF4 was genetically stable, although spontaneously occurring plasmidless segregants...

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Plasmid transfer from Escherichia coli to Bacteroides fragilis: differential expression of antibiotic resistance phenotypes.

A unique shuttle plasmid, pDP1, has been constructed to mediate gene transfer between Escherichia coli and the Gram-negative anaerobe Bacteroides fragilis. pDP1 contains the pBR322 replicon and the Bacteroides clindamycin resistance plasmid pCP1 linked to the transfer origin of the broad host range plasmid RK2. pDP1 can be transferred from E. coli to B. fragilis by the RK2 conjugation system ev...

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P-Lactamase-Mediated Imipenem Resistance in Bacteroides fragilis

Imipenem has excellent antimicrobial activity owing in part to ,-lactamase stability. We found that only 2 of over 350 Bacteroides fragilis group clinical isolates were resistant to imipenem, with an MIC of more than 16 ,ug/ml. These two isolates from the Tufts Anaerobe Laboratory (TAL) were resistant to all other P-lactam agents tested. The organisms were able to inactivate imipenem in broth c...

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ژورنال

عنوان ژورنال: The Journal of Antibiotics

سال: 1990

ISSN: 0021-8820,1881-1469

DOI: 10.7164/antibiotics.43.1302